Photo-ptyr-scaffold
WebMar 28, 2024 · Furthermore, the lifetime of the photo-pTyr-scaffold probe in a 96-well microplate was significantly extended from 1 week up to 1 month. More importantly, by combining with high-flow nano LC ... WebAug 16, 2024 · Indeed, as regarding the analysis of pTyr signaling in human cells, Chu at el. [65] have recently developed a trifunctional chemical probe termed photo-pTyr-scaffold, …
Photo-ptyr-scaffold
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WebFind the latest published documents for phosphotyrosine signaling, Related hot topics, top authors, the most cited documents, and related journals WebJul 18, 2024 · Europe PMC is an archive of life sciences journal literature.
WebJul 11, 2013 · Cell-surface receptors frequently use scaffold proteins to recruit cytoplasmic targets, but the rationale for this is uncertain. Activated receptor tyrosine kinases, for example, engage scaffolds such as Shc1 that contain phosphotyrosine (pTyr)-binding (PTB) domains. Using quantitative mass spectrom … WebPhosphotyrosine (pTyr)-dependent protein complexes are key machinery for regulating cancer signaling. We developed the Photo-pTyr-scaffold approach for unbiasedly …
WebJul 8, 2024 · The Photo-pTyr-scaffold-based forward-phase protein array could be generically applicable for exploring the dynamic pTyr signaling complexes in various … WebLow-abundance phosphotyrosine (pTyr)-mediated signaling protein complexes play critical roles in cancer signaling. The precise and comprehensive profiling of these pTyr-mediated protein complexes remains challenging because of their dynamic nature and weak binding affinity. Taking advantage of the SH2 domains modified with trifunctional chemical …
WebPhosphotyrosine (pTyr)-regulated protein complexes play critical roles in cancer signaling. The systematic characterization of these protein complexes in tumor samples remains a challenge due to their limited access and the transient nature of pTyr-mediated interactions. We developed a hybrid chemical proteomics approach, termed Photo-pTyr-scaffold, by …
WebLow-abundance phosphotyrosine (pTyr)-mediated signaling protein complexes play critical roles in cancer signaling. The precise and comprehensive profiling of these pTyr … boxthomasWebBy assembling the photo-pTyr-scaffold probe into a 96-well microplate, we achieved steric hindrance-free photoaffinity capture of pTyr signaling complexes, selective enrichment under denaturing conditions, and efficient in-well digestion in a fully integrated manner. EGFR signaling complex proteins could be efficiently captured and identified ... gut martelshofWebApr 26, 2024 · Compared with the previously developed photo-pTyr-scaffold strategy, by assembling the tyrosine phosphorylation (pTyr) binding domain through covalent NHS … boxt historyWebPhoto-pTyr-scaffold approach for unbiasedly capturing and ex-ploring weak and dynamic pTyr protein complexes. By utilizing the Src kinase Src homology 2 superbinder with nanomolar binding affinity, Photo-pTyr-scaffold showed superior sensitivity for profiling native pTyr protein complexes in cancer cells and breast tumor samples. box thomsonWebAug 6, 2024 · The Photo-pTyr-scaffold-based forward-phase protein array could be generically applicable for exploring the dynamic pTyr signaling complexes in various … gut marthalenWebFurthermore, the lifetime of the photo-pTyr-scaffold probe in a 96-well microplate was significantly extended from 1 week up to 1 month. More importantly, by combining with … gut math articleWebThe Photo-pTyr-scaffold approach may become a generic tool for profiling dynamic pTyr signaling complexes readily in clinical relevant samples. [dataset license: CC0 1.0 … gut matheshof johannes schmidt