Web18 Feb 2016 · SMMC-7221 cells were transferred into 24-well plate, and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing antibiotics and 10 % (v/v) fetal … Web15 Aug 2012 · The SMMC-7221 hepatoma cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and confirmed as not infected with HBV. Thirty-three HCC tissue samples were obtained from 29 male and 4 female patients between 2006 and 2007 at the Institute of Hepatobiliary Surgery, Southwest Hospital (Chongqing, …
Hypoxia promotes EMT of HCC cells via inducing Twist1 expression
Web8 Jul 2024 · To determine the role of miR-346 in liver cancer, real-Time PCR was performed to show that miR-346 was overexpressed in five liver cancer cell lines, including MHCC-97H, SMMC-7221, HepG2, Huh-7, and Hep3B, compared with the normal liver cell lines THLE-2 and THLE-3 (Fig. 1). This result demonstrated that, compared with normal human liver … WebD Morphological changes of the four groups of SMMC-7221 and HepG2 cells. E The expression levels of EMT markers were detected by Western blotting in SMMC-7221 and HepG2 cells with NQO1... dates in excel pivot table format issues
Expression Profiling Identifies Circular RNA Signature in …
Web20 Nov 2024 · The expression of TUFT1 in SMMC-7221 cell lines was down-regulated by lentiviral vector. Cell proliferation assay, clonogenic assay, cell apoptosis assay and cell … Web16 Oct 2024 · They were subcutaneously inoculated with SMMC-7221 cells (5 * 10 6) stably expressing control lentivirus and shPYCR1 lentivirus, respectively. After the diameter of tumor was larger than 5 mm, tumor volume was determined twice per week. The tumor volume was calculated as V = (width 2 * length)/2. The bioluminescence imaging was … Web22 Feb 2024 · Two micrograms of total RNA extracted from LO2, HepG2, and SMMC-7221 cells were reverse-transcribed using a GoScript reverse transcription kit (Promega) follow the manufacturer’s instructions. Quantitative RT-PCR was performed following procedures described previously and the expression levels of each gene were normalized to GADPH … date single moms near me