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Smmc 7221

Web18 Feb 2016 · SMMC-7221 cells were transferred into 24-well plate, and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing antibiotics and 10 % (v/v) fetal … Web15 Aug 2012 · The SMMC-7221 hepatoma cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and confirmed as not infected with HBV. Thirty-three HCC tissue samples were obtained from 29 male and 4 female patients between 2006 and 2007 at the Institute of Hepatobiliary Surgery, Southwest Hospital (Chongqing, …

Hypoxia promotes EMT of HCC cells via inducing Twist1 expression

Web8 Jul 2024 · To determine the role of miR-346 in liver cancer, real-Time PCR was performed to show that miR-346 was overexpressed in five liver cancer cell lines, including MHCC-97H, SMMC-7221, HepG2, Huh-7, and Hep3B, compared with the normal liver cell lines THLE-2 and THLE-3 (Fig. 1). This result demonstrated that, compared with normal human liver … WebD Morphological changes of the four groups of SMMC-7221 and HepG2 cells. E The expression levels of EMT markers were detected by Western blotting in SMMC-7221 and HepG2 cells with NQO1... dates in excel pivot table format issues https://mommykazam.com

Expression Profiling Identifies Circular RNA Signature in …

Web20 Nov 2024 · The expression of TUFT1 in SMMC-7221 cell lines was down-regulated by lentiviral vector. Cell proliferation assay, clonogenic assay, cell apoptosis assay and cell … Web16 Oct 2024 · They were subcutaneously inoculated with SMMC-7221 cells (5 * 10 6) stably expressing control lentivirus and shPYCR1 lentivirus, respectively. After the diameter of tumor was larger than 5 mm, tumor volume was determined twice per week. The tumor volume was calculated as V = (width 2 * length)/2. The bioluminescence imaging was … Web22 Feb 2024 · Two micrograms of total RNA extracted from LO2, HepG2, and SMMC-7221 cells were reverse-transcribed using a GoScript reverse transcription kit (Promega) follow the manufacturer’s instructions. Quantitative RT-PCR was performed following procedures described previously and the expression levels of each gene were normalized to GADPH … date single moms near me

Suppression of stromal interaction molecule 1 inhibits SMMC7721 …

Category:Lepipyrrolins A-B, two new dimeric pyrrole 2 ... - ScienceDirect

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Smmc 7221

Recombinant Balsamin induces apoptosis in liver and

Web10 Jan 2024 · SMMC-7221 and another HCC cell line Huh-7, which showed relatively low AGO2 protein expression, were transiently transfected with pCMV3-AGO2 and control pCMV3 vector, respectively. Increased production of AGO2 in both SMMC-7221 and Huh-7 was detected by western blot analysis 48 hours post transfection (Figures 6(a) and 6(g)). …

Smmc 7221

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Web1 Jul 2024 · Furthermore, compound 1 was evaluated for its cytotoxic activity against five human tumor cell lines (HL-60, SMMC-7221, A549, MCF-7, and SW480) in vitro, and displayed selective cytotoxicity against SMMC-7721 with IC 50 value of 16.78 ± 0.49 μM. Graphical abstract. Web13 Jul 2024 · Some researchers also extracted the active components from Bupleurum spp. callus (BSW-ws) and administered SMMC-7221 to cultured liver cancer cells at concentrations of 10, 20, 30, 40, 60, 80, 100 μg•mL-1 ; MTT method was used to detect the cytotoxic activity of BSW-ws, and the effect of BSW-ws on cell apoptosis was detected by …

WebThe cytotoxicity induced by brucine from the seed of Strychnos nux-vomica proceeds via apoptosis and is mediated by cyclooxygenase 2 and caspase 3 in SMMC 7221 cells. To … Web12 Dec 2024 · In this manuscript, a series of α-mangostin derivatives were synthesized and then all compounds were evaluated for cytotoxic activities against five human cancer cell …

WebSMMC-7721 is a human hepatocellular carcinoma (HCC) cell line that was established from a liver tumor of a Chinese patient. HCC is the most common type of liver cancer, and the … WebThe results showed that brevilin A reduced cell viability and invasion in HepG2 and SMMC-7221 cells. The apoptotic rates of HepG2 and SMMC-7221 cells treated with brevilin A …

WebXylitol/selenite is a promising anti-cancer drug to induce apoptosis in SMMC-7221 cells. It may regulate the apoptosis through the co-action of multiple mechanisms related to GSH …

WebSMMC-7721 cells were cultured for 24 h respectively under the normal oxygen envi- ronment and hypoxic environment, and cells adhering to the wall in the 6-well plate were washed using pre-heated phosphate buffer sali- ne (PBS) for three times. Thereafter, cells were transferred into the radioimmunoprecipitation assay (RIPA) for lysis. bizz johnson trail shuttleWeb14 Jan 2024 · SMMC-7721 cells transfected with STOML2-Flag were lysed in RIPA buffer and then loaded to Flag antibody and protein A/G agarose beads (Santa Cruz, sc-2003), the beads were washed with RIPA buffer for 5 times. Proteins complex was eluted using loading buffer, separated on SDS-PAGE gel and silver stained. dates in german formatWeb18 Jun 2014 · (A, B and C) Anoikis analysis is shown for (A) SMMC-7221 cells expressing either a control vector or two different clones expressing AEG-1; (B) MHCC-97H cells expressing either NC siRNA control or two different siRNAs against AEG-1; and (C) HCC-LM3 cells expressing NC or two different siRNAs against AEG-1. Cells were trypsinized and re … dates in excel chartWebA copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2024; you can also visit the original URL.The file type is application/pdf. bizzleberry twitterWeb20 Feb 2024 · In SMMC7721 cells, quercetin at 20 µM leads to cleavage of caspase-3, and procaspase-3 into a p20 intermediate, thereby leading to liver cancer cell apoptosis [ 51 ]. A study has shown that it affects enzymatic activity of caspase-8 after 4–18 h of incubation in all quercetin concentrations. bizzle builds repositoryWebReactive oxygen species (ROS) play an important role in cell death induced by many different stimuli. Direct exposure of human hepatoma cell line SMMC-7221 to hydrogen peroxide (H 2 O 2) can induce apoptosis characterized by morphological evidence and fragmentation of DNA assayed by terminal deoxynucleotidyl transferase assay (TUNEL assay).Analysis of … bizzjoy inverclydeWeb27 Mar 2012 · The human SMMC-7221 human HCC cell line was used. Immunofluorescence was used to investigate the expression levels of CD147. Anoikis-induced cell death was assessed using trypan blue exclusion. In the present study, the results showed that SMMC-7721 HCC cells exhibited significant morphological changes … date singer featherweight