Spinach fluorescence
WebMay 11, 2015 · Spinach riboswitches use Spinach, an RNA aptamer that binds and activates the fluorescence of an otherwise nonfluorescent small-molecule fluorophore. Drawing upon structural insights into the … WebOct 29, 2014 · The fluorescence intensity was normalized to that of the unmodified Spinach aptamer Sp (500 nM), which corresponds to the sum of the concentrations of Sp-IC and Sp-IF. The linearity of the Spinach fluorescence was confirmed for the Sp concentrations between 0 to 500 nM ( Supporting Information Figure S2 ).
Spinach fluorescence
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WebMar 3, 2012 · Here we describe fluorescence imaging of small molecules using RNA. These RNA-based sensors comprise a ligand-binding RNA aptamer and Spinach, an aptamer that binds and switches on the fluorescence of a small molecule fluorophore. WebAug 1, 2013 · Spinach is a 98-nt RNA aptamer that binds and switches on fluorescence of a dye resembling the chromophore of GFP ( Figure 1 a) [ 4••, 5 ]. The dye, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI), was synthesized alongside several related dyes and chosen on the basis of photophysical properties [ 4 ••].
WebOct 27, 2013 · Spinach fluorescence in vivo is improved by inserting Spinach into the tRNA Lys3 sequence 3, 5, which acts as a folding scaffold 6. In the case of Spinach, folding … WebMar 10, 2024 · The tRNA Lys -Spinach-tRNA Lys, once delivered into “chloroplast-free” onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results …
WebMar 3, 2012 · Spinach contains three stem-loops encircling a central loop (fig. S1A–B) . Mutagenesis revealed that one stem-loop has an essential structural role in Spinach … WebTo elucidate the molecular basis of Spinach fluorescence, we solved the cocrystal structure of Spinach bound to its cognate exogenous …
WebTo elucidate the molecular basis of Spinach fluorescence, we solved the cocrystal structure of Spinach bound to its cognate exogenous chromophore, showing that Spinach activates the small molecule by immobilizing it between a base triple, a G-quadruplex and an unpaired G. Mutational and NMR analyses indicate that the G-quadruplex is essential for …
WebFluorogenic RNA aptamers are short nucleic acids able to specifically interact with small molecules and strongly enhance their fluorescence upon complex formation. Among the … elpony スマホケースWebSep 25, 2024 · In vitro Spinach fluorescence . 111. Prior to delivery of the Spinach RNA aptamer, or Spinach-tagged RNAs into plant cells . 112. and tissues, we tested if flanking a plant tRNA at both 5’ and 3 ... elpsc21b エプソンWebJul 29, 2011 · Spinach is different from GFP in that it exhibits considerable resistance to photobleaching, and fluorescence is observed shortly after Spinach transcription in cells, … elpsc24 エプソンWebFeb 22, 2024 · In its standard form, spinach folds and binds 3,5-difluoro-4-hydroxybenzylidene imidazolinone, which fluoresces green when bound. An analogous hachimoji aptamer with nonstandard nucleotides placed strategically to avoid disrupting its fold ( Fig. 4) was prepared by transcribing hachimoji DNA using the FAL variant of RNA … elpsc24 カタログWeb(a) Spinach is an RNA aptamer that binds a small-molecule dye called DFHBI (green ball). Both DFHBI and Spinach are nonfluorescent until binding occurs and activates the fluorescence of the Spinach-DFHBI complex. Stem loop 3 of Spinach can tolerate insertion of additional sequences, and it is the region that is modified to generate sensors. elpsc32 エプソンWebJun 18, 2014 · Spinach is a recently reported in vitro-evolved RNA mimic of GFP, which as genetically encoded fusions makes possible live-cell, real-time imaging of biological RNAs … elpmb46 エプソンWebDec 19, 2013 · Spinach is a 98-nt-long RNA aptamer that binds to and switches on the fluorescence of 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI), a small molecule that resembles the chromophore of GFP 1. elpsc24 スクリーン